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KMID : 0379119990270010039
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Korean Journal of Mycology
1999 Volume.27 No. 1 p.39 ~ p.43
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Phylogenetic Study of Ganoderma spp. Based on the DNA Sequences in ITS 2 Region
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류진창/Ryu, Jin Chang
성재모/박동석/고승주/Sung, Jae Mo/Park, Dong Suk/Go, Seung Joo
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Abstract
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The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Ganoderma spp. were amplified using polymerise chain reaction (PCR) and sequenced. Sequences from 9 species including Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum and Inonotus xeranticus as an out-group were compared. The spacer regions of them were 247∼257 nucleotides in length and contained partial sequences of 5.8S and 25S gene. The reciprocal homologies of each ITS II sequence of the species were in the range of 70∼100 % except outgroup species, I. xeranticus. According to the analysis of ITS II sequences, Ganoderma spp. constructed 5 clusters. Ganoderma lucidum isolates were to be divided into two groups. One group was consisted of isolates from South Korea. The other group comprised isolates from UK. G. lucidum isolates belonging to the grnup I were closely related with G. tsugae. These results suggested that G. lucidum from Korea should be G. tsugae, otherwise G. tsugae was to be synonym of G. lucidum.
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